The significance line and symbol can be used to show that the two datasets are significantly different from one another. These lines usually have a symbol (eg *) or text (eg P<0.05) to provide additional information to the reader. I’m talking about the horizontal, and sometimes zig-zag, lines that are added onto graphs to indicate when significant differences are present between datasets. I don’t believe the correct term is significance lines but I am going to use this hereafter. Kindly ignore the opportunity amount as shown above which has been defaulted to USD 500.In this tutorial I will explain how to use GraphPad Prism to add lines with text to depict statistical significance between datasets on graphs.
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